70 patients with severe oligozoospermia and azoospermia and Y chromosome microdeletion in patients with.docVIP

 PAGE \* MERGEFORMAT 8 70 patients with severe oligozoospermia and azoospermia and Y chromosome microdeletion in patients with Of: Wang Xiliang, Dong-Mei Hao, Hou Kaibo, Zhang Ning, Yan Su Wen [Abstract] Objective: To investigate the severe oligozoospermia and azoospermia and Y chromosome microdeletions. Methods: 70 patients with normal chromosomes severe oligozoospermia and azoospermia were detected by multiplex PCR Y chromosome AZF gene family AZFa , AZFb, AZFc three regions in the six sequence tag sites (sequence tagged sites, STS) microdeletion, 20 cases of normal sperm were used as control. Results: 39 patients with severe oligozoospermia were found in 5 cases of Y chromosome microdeletions there , are the AZFc deletion, defect rate was 12.82% (5 / 39); 31 cases of azoospermia in the presence of 6 cases found in Y chromosome microdeletions, including 2 cases of missing AZFb + AZFc, AZFb deletion in 3 cases, AZFc deletion in 1 case , loss rate was 19.35% (6 / 31) .20 cases of patients with normal semen Y chromosome microdeletions were not found. Conclusion: Y chromosome microdeletions in male spermatogenesis is caused by one of the common obstacles. [Keywords:] severe oligozoospermia; azoospermia; Y chromosome microdeletions AbstractNFDEAObjective: To assess the relationship between severe oligozoospermia or azoospermia and Y chromosomal microdeletions.Methods: Seventy chromosome normal infertile men with severe oligozoospermia or azoopermia were screened by multi-polymerase chain reactions with 6 sequence tagged sites in AZFa, AZFb, AZFc region, and 20 normospermic men as controls.Results: 12.82% (5 / 39) of microdeletions were detected in 39 severe oligozoospermia, and microdeletion type was AZFc.19.35% (6 / 31) of microdeletions were detected in 31 azoospermia, and microdeletion types were AZFb + AZFc, AZFb and AZFc.Microdeletions were not detected in 20 normospermic men.Conclusion: Y chromosome microdeletion is one of the causes which result