Adriamycin on Gfi 1 expression in K562 cells and its relationship with apoptosis-related genes Relationship.docVIP

 PAGE \* MERGEFORMAT 12 Adriamycin on Gfi 1 expression in K562 cells and its relationship with apoptosis-related genes Relationship Author: Chang-Wei SUN Han-ying Huang Min-Cheng Zhang Jian-Feng Zhou Abstract This study investigated the role of adriamycin in vitro effects on K562 cells and cancer cells, gene Gfi  1 and the associated changes in gene expression of apoptosis mechanism. With different concentrations of doxorubicin in K562 cells, 24 hours, and then apply the DNA gel electrophoresis and flow cytometry detection of apoptosis in K562 cells; by RT  PCR, flow cytometry Gfi  1, Bcl  2, bax gene and protein expression changes. The results showed that: when the concentration of adriamycin 0,0.5,2.0 mg / L the role of K562 cells, 24 hours, apoptosis increased, showing a typical fracture electrophoresis DNA bands; the same time, when the concentration of ADM increased from 0.5 to 2.0 mg / L,, Gfi  1 expression was decreased, bax expression increased; Bcl  2 expression in the ADM 0.5-2.0 mg / L did not change significantly between, mRNA and protein levels there was no statistical difference; as adriamycin concentration is higher than 2.0 mg / L, the apoptosis rate is not increased but decreased, and necrosis occurs. Conclusion: Be sure the concentration of doxorubicin could induce apoptosis of K562 cells, the apoptotic rate changes and the expression of a certain concentration of adriamycin dose-dependent relationship between adriamycin-induced apoptosis in K562 cells may be related to inhibition Gfi  1 gene Expression and activation of bax gene expression in a certain relationship. Keywords: Doxorubicin K562 cells; apoptosis; Gfi  1; Bcl  2; bax Effect of Adriamycin on Gfi1 Expression in K562 Cells and Its Relationship with the Relevant Apoptotic Genes Abstract The study was purposed to explore the effect of adriamycin (ADM) on K562 cells in vitro and the mechanism of expression changes of relevant apoptotic genes an