Annona lactones on human hepatoma cell line HepG2 on the role of.docVIP

 PAGE \* MERGEFORMAT 7 Annona lactones on human hepatoma cell line HepG2 on the role of Of: Ren Fengmei, Kuai Yu-Hua, Zhang Xiaochun, Peng Haiyan, Wang Mingyan, Zhang Yong [Abstract] Objective Fan acetogenins AS-4 in vitro on human hepatoma cell line HepG2 growth inhibition. Methods The different concentrations of AS-4 group (6.25,12.5,25,50 μg / mL), cisplatin-positive the control group (25 μg / mL) and blank control group, routine culture 24,48 h, the MTT colorimetric test and phase contrast microscopy. Results AS-4 can inhibit the growth of HepG2 cells, the maximum inhibitory effect reached 91.68%, the time and have cytotoxicity and dose dependent, 48 h IC50 is 6.67 μg / mL. Conclusion Fan acetogenins AS-4 in vitro on human hepatoma cell line HepG2 with killing effect, showing a strong cytotoxic activity. [Keywords:] Fan acetogenins, HepG2 liver cancer cell lines, methyl thiazolyl tetrazolium Abstract: Objective To observe the inhibitory action of annonaceous acetogenins AS-4 on human liver cancer cell line HepG2 in vitro. Method Using different concentration of annonaceous acetagenins group (6.25, 12.5, 25, 50 μg / mL), control group (25 μg / mL) of masculine (DDP), control blank group, after routine culture of 24 or 48 hours, the effect of annonaceous acetogenins AS-4 on HepG2 was analyzed by colorimetry assay (MTT) and inverted phase-contrast microscope observing. Result AS -4 had the best inhibitory effect on HepG2, the highest inhibitory rate was 91.68%, and the effect was time-dosage dependent, and the 48 h IC50 was 6.67 μg / mL. Conclusion AS-4 has lethal effect on HepG2 in vitro, and shows obvious cytotoxic effect. Keywords:: annonaceous acetogenins, human liver cancer cell line HepG2, MTT Custard apple (Annona squamosa Linn.) For the Annonaceae (Annonaceae) Annona genus (Annona) plant in China, Zhejiang, Yunnan and other provinces are growing. To study the physiological active components, we have the chemical composition of seeds