Bone and cartilage tissue engineering construction.docVIP

 PAGE \* MERGEFORMAT 7 Bone and cartilage tissue engineering construction Keywords:: bone; cartilage; cell; Tissue Engineering 0 Introduction of human disease due to congenital and acquired bone and cartilage caused by defects or deformities, the need to use the ideal completion of the repair or replacement materials, bone and cartilage repair and reconstruction. Today there is no biological characteristics of a repair material in side can reach the level of autologous tissue transplantation. Therefore, using tissue engineering, and construction of its own cell-derived autologous tissue has become an important topic in biomedical [1,2]. I subjects by the National Health Fund and the Army Medical Research Fund for tissue-engineered bone and cartilage research, now part of the research done to introduce. 1 Materials and methods 1.1 bone tissue engineering research experiments using nude mice and New Zealand rabbits as the experimental model. 1.1.1 Preparation of scaffold materials to take New Zealand white rabbits of the knee joint, washing, rinsing to remove bone marrow, using degreasing, de-mining, to acidic protein and other chemical treatment methods, after freeze-dried after treatment, preparation into the spongy bone matrix. Bone spongy matrix with a porous network structure. 1.1.2 Cell Isolation and culture of rabbit bone marrow stromal cells obtained by in vitro induction of differentiation into bone cells cultured in vitro amplification of biodegradable hydrogel after mixing. 1.1.3 cell / scaffold complexes the formation of the 5 × 106 * mL-1 osteoblasts seeded in cancellous bone matrix scaffold on the surface line of calcium ions curing, cultured 48 h. Then cells / scaffold complexes sik into nude mice and New Zealand, under Rabbit. to pure cancellous bone matrix grafts served as controls. 1.2 Construction of tissue-engineered cartilage 1.2.1 cells, the choice of carrier materials