Bortezomib inhibited NF-B activation on cell proliferation LOVO.docVIP

 PAGE \* MERGEFORMAT 17 Bortezomib inhibited NF-B activation on cell proliferation LOVO Of: Zhou Weimin, Jin Peiying, Ping Jin-liang, Qian Fu beginning, Zhang Lei [Abstract] Objective?: Of bortezomib on the nuclear factor-B (NF-B) activation and its effects on human intervention LOVO cells. Methods: The human colon cancer cells LOVO treated with different concentrations of bortezomib to Western blot and ELISA were used to detect IB @ and the expression of NF-B; by MTT method and Annexin V-FITC/PI color markers were detected by cell proliferation and apoptosis. Results: Bortezomib role LOVO cells, cytoplasmic NF-B and IB @ level gradually increased, while levels of nuclear NF-B was significantly lower (P? lt;0.01), and a dose-dependent, the most significant effect of high concentration (P? lt;0.01); bortezomib can inhibit the proliferation of LOVO cells, and a dose and time dependent. With the increase in the concentration of bortezomib, apoptosis was significantly increased (P? lt;0.01). Conclusion: B bortezomib by inhibiting the degradation of IB @ effective intervention NF-B activation and inhibition of cell proliferation and induced LOVO apoptosis. [Keywords:] bortezomib; colon cancer; nuclear factor-B; LOVO; apoptosis Abstract:? Objective: To investigate the influences of LOVO cells of the intervention of nuclear factor-B (NF-B) activation by bortezomib on the proliferation. Methods: LOVO cells was cultured in vitro and treated with different concentrations of bortezomib. The expressions of IB @ and NF-B were analyzed by western blot and enzyme linked immunosorbent assay, respectively. The changes of cell growth and apoptosis were investigated by MTT assay and Annexin-V/PI double dyeing assay, respectively. Results: In bortezomib-treated cells, the expressions of IB @ and NF-B in cytopalsm were obviously upregulated, while the level of NF-B in nucleus was sharply downregulated in a dose-dependent manner (P? lt;0.01), which was the most effective