Chuanxiong inter-simple sequence repeat polymorphism and amplified fragment length polymorphism molecular markers Comparative Study of Polymorphism.docVIP

 PAGE \* MERGEFORMAT 15 Chuanxiong inter-simple sequence repeat polymorphism and amplified fragment length polymorphism molecular markers Comparative Study of Polymorphism Author: Chen to Zhen, Wang L, Ma Yu-ying, Zhang Zheng-Yin, Fan Zhe-ren, Tang Lin [Abstract] Objective To search for an efficient molecular marker Chuanxiong. Methods Chongzhou Dujiangyan, Sichuan, Xindu 3 origin Chuanxiong as material, the application of 100 simple sequence repeat polymorphisms between the district (ISSR) primers, 64 pairs of amplified fragment length polymorphism (AFLP) primer pairs of 30 individuals Chuanxiong Screening for polymorphisms. The results of screening to simple sequence repeat polymorphisms of inter-specific primers, a total of 10 amplified fragment length polymorphism-specific primers, six pairs; respectively, 106 and 256 roads lead to amplified band, the percentage of polymorphic bands were 22.64% and 22.27%. Labeling index average of AFLP detection efficiency (1.78) was significantly higher than the ISSR (0.42). Two kinds of tags based on genetic similarity values of cluster analysis there are some differences, but with the Mantel test of the two methods of detecting the same degree of genetic correlation analysis showed that among them there is a significant correlation (r = 0.6975, p = 0.014). Conclusion Chuanxiong have low genetic diversity, AFLP is more efficient in the ISSR. [Keywords:] Chuanxiong inter simple sequence repeat polymorphisms of amplified fragment length polymorphism Chuan Xiong Ligusticum chuanxiong Hort. To Umbelliferae Ligusticum of perennials, is a famous traditional Chinese herbal medicines. In order to root for medicinal, with a blood circulation of qi, Qufeng analgesic effect, for irregular menstruation, amenorrhea dysmenorrhea, Zhengjia abdominal pain, Sternocostal tingling, headache, rheumatism Bi Tong et al [1]. In China, widely distributed in Sichuan, Jiangxi, Yunnan, Jilin, Gansu, Guizhou and Jiangsu provinces. Sichuan Road,