Cnidium supercritical carbon dioxide extract of Determination of the active ingredient.docVIP

 PAGE \* MERGEFORMAT 9 Cnidium supercritical carbon dioxide extract of Determination of the active ingredient [Abstract] Objective To determine the Cnidium supercritical carbon dioxide (CO2) extracts Osthol and total coumarin content. Methods HPLC method and UV method Osthol in Osthol and total coumarin content were determined. Results osthol range of 0.275 ~ 2.20 μ g in a good linear relationship (r = 0.999 8); recovery was 100.02%, RSD of 1.05%. Total coumarins in the 2.20 ~ 11.00 μ g * ml-1 range of a good linear relationship (r = 0.999 7); recovery was 99.65%, RSD of 1.91%. Conclusion The method is accurate, sensitive, specific, can be used as the quality of the extracts of control methods. [Keywords:] Cnidium extracts of the total coumarins Osthol high-performance liquid chromatography; Ultraviolet - visible spectrophotometry Cnidium supercritical CO2 extract of the Umbelliferae Cnidium Cnidium monnieri (L.) Cuss.’s Dried ripe fruit by supercritical CO2 extraction of the resulting liquid extract containing the main coumarin class and volatile oil composition, which is the coumarins Osthol one of the main active ingredient, inhibits platelet aggregation, anti-virus, anti-mutagenic, anti-allergic, as well as inhibiting platelet aggregation and other pharmacological activities [1]. New pharmacological studies [2] showed that the total Osthol coumarin on osteoporosis prevention and treatment of significant effect. High and low levels of its active ingredients have a major impact on their medical effectiveness. HPLC method used in this experiment, UV Determination of Osthol, the total content of coumarin on the Supercritical CO2 extract of Cnidium quality control studies, the use of samples to provide a viable quality control methods, while anti-bone osteoporosis new drug research experimental data. An instrument and reagent Shimadzu LC-20A-based high-performance liquid chromatography, UV detector; UV-1800PC UV - VIS spectrophotometer (Shanghai Spectrum