Cuspidatum Angelica extraction of Analysis_0.docVIP

 PAGE \* MERGEFORMAT 8 Cuspidatum Angelica extraction of Analysis [Abstract] Objective To determine cuspidatum, Angelica best extraction of condition. Method to extract yield and the amount of Polydatin leaching index, the main factors affecting the extraction were optimized. Results The optimum extraction conditions were: plus 60 % ethanol, extracting 2 times, plus the amount of alcohol were 10,8 times, extraction times were 1.5,1.0 h. Conclusion optimal extraction process can provide the experimental basis for industrial production. [Keywords:] orthogonal design, extraction of, giant knotweed, angelica, Polydatin Cuspidatum, Angelica is commonly used traditional Chinese medicine, mainly for the chemical composition of Polygonum cuspidatum resveratrol class [1], anthraquinone. Polydatin (resveratrol glucoside) is the main active ingredient in Polygonum, has significant vasodilator effect on coronary, cerebrovascular, pulmonary vascular, liver, blood vessels have expanded the role of vascular smooth muscle cells and calcium , pH with a two-way adjustment. In preparation cuspidatum, Polydatin its active ingredient can be used a certain concentration of alcohol withdrawal. The orthogonal design [2-3] approach to ethanol as extraction solvent, prescription of the preparation The main Smell cuspidatum, Angelica alcohol extraction conditions were optimized for industrial production of reference. An instrument and reagent LC-10AT liquid chromatograph, SPD-10A UV detector, Apollo column: Apollo C18-A (250 mm × 4.6 mm, 5 μm), N3000 chromatography workstation, electronic analytical balance (AY120 Shimadzu 0.1 mg ~ 120 g); constant temperature water bath (DK-SZZ-type). cuspidatum, Angelica sinensis were purchased from Hunan Sunshine Co., Ltd. Chinese Herbal Medicine, herbs are qualified by examination. Polydatin (batch 111575-200502) from China Pharmaceutical Biological Products available. Acetonitrile for chromatography pure, distilled water, water and other