Determination of cyromazine in greasy wool and ring fipronil.docVIP

 PAGE \* MERGEFORMAT 15 Determination of cyromazine in greasy wool and ring fipronil Study: Fan Yuan, animal husbandry, Yu Xuejun Gu Xiaojun Yan Yi Qiu Yajun Chen Shubing Home Zhan Jia Jie Chen Jun Huang Shaotang He drizzle [Abstract] A high performance liquid chromatography (HPLC determination of greasy wool in the ring fipronil cyromazine and the methods and HPLC-MS/MS confirmation methods. Samples with 80 mL 1% trichloroacetic acid solution, ultrasonic extraction, MCX column purification, Hypersil NH2 column chromatography, water - acetonitrile as mobile phase gradient elution, 214 nm detection, HPLC-MS/MS confirmed. in the positive ion electrospray ionization (ESI + mode, loop fipronil [M + H] + and 2 were the main features of ion m / z 191.0,150.0 and 163.0, cyromazine, [M + H] + and the two major characteristic ions were m / z 167.0,85.0 and 125.0. 0.05 ~ 5.0 mg / L range, and ring fipronil cyromazine have good linear correlation coefficient of 0.9999. The detection limit of cyromazine was 0.02 mg / kg, Central fipronil was 0.01 mg / kg . The average recovery rate: 95.0% cyromazine, 99.9%, Central fipronil 83.6% to 92.2%. [Keywords:] cyromazine, fipronil ring, greasy wool, residue analysis, HPLC, high performance liquid chromatography - tandem mass spectrometry Abstract A method for the determination of cyromazine and dicyclanil residues on greasy wool was developed with HPLC and confirmed with HPLC-MS/MS.The cyromazine and dicyclanil residues on greasy wool were extracted with 1% trichloroacetic acid solution with ultrasonic, and cleaned up by MCX SPE column.The HPLC separation was performed on a Hypersil NH2 using water-acetonitrile (V / V) as the mobile phase with gradient elution and detected at the wavelength of 214 nm.The corroboration method of HPLC-MS/MS was used with electro-spray ionization of positive ion mode.The [M + H] + and characteristic ions of dicyclanil were m / z 191.0,150.0 and 163.0, and cyromazine were m / z 167.0,85.0 a