Different collection period of the Jiangxi ursolic acid content of Gardenia.docVIP

 PAGE \* MERGEFORMAT 11 Different collection period of the Jiangxi ursolic acid content of Gardenia Author: TANG Can-Yun-Peng Li Zhang Yanyan Luo Wen Pu Qing-Rong Zhao early [Abstract] Objective To determine the optimum harvest period of gardenia, Jiangxi, Jiangxi, gardenia to ensure quality. Methods using high performance liquid chromatography with ursolic acid as a chemical reference substance, the stationary phase for the UltimateTM C18 bonded silica gel column (4.6 mm * 250 mm, 5 μ m), mobile phase was methanol: water: acetic acid: triethylamine (90:10:0.03:0.06), flow rate 0.6 ml * min-1, temperature 25 ℃ , wavelength 210 nm Determination of ursolic acid in Jiangxi gardenia. The results of ursolic acid in the range of 0.1 ~ 3.0 μ g in peak area A with the quality of M showed a good linear relationship, the regression equation: A =- 12 954.88 452 850.96 M, r = 0.999 6, n = 7; 3 groups Jiangxi Gardaniajas minoides Ellis in the content of ursolic acid, respectively (0.591 0 ± 0.003 944) mg * g-1 medicines, RSD = 0.667 8%, n = 3; (0.576 0 ± 0.005 033) mg * g-1 medicines, RSD = 0.873 7%, n = 4; (0.544 4 ± 0.010 96) mg * g-1 medicines, RSD = 2.039%, n = 3; 3 groups, Jiangxi Red Gardenia content of ursolic acid, respectively (0.361 6 ± 0.000 360) mg * g-1 medicines, RSD = 0.100%, n = 3; (0.355 ± 0.006 250) mg * g-1 medicines, RSD = 1.763%, n = 5; (0.381 6 ± 0.002 835) mg * g-1 medicines, RSD = 0.743 1%, n = 3; precision experimental RSD = 1.754%, good precision instruments; stability of the experimental RSD = 2.493%, measured at 3.5 h of ursolic acid is stable; Jiangxi Gardaniajas minoides Ellis repeated experiment RSD = 0.941 1%, Jiangxi red gardenia repeated experiment RSD = 0.779 2%, average recovery was 101.9%. Conclusion of ursolic acid in Jiangxi Gardaniajas minoides Ellis was significantly higher than that in Jiangxi red gardenia, select a reasonable period of harvesting is very important for the quality of Jiangxi gardenia, Gardenia for oth