Effect of rosiglitazone on CXCL16 induced the proliferation of vascular smooth muscle cells.docVIP

 PAGE \* MERGEFORMAT 11 Effect of rosiglitazone on CXCL16 induced the proliferation of vascular smooth muscle cells [Abstract] Objective To study rosiglitazone on CXCL16-induced proliferation of vascular smooth muscle cells. Methods MTT was observed in vitro CXCL16 on the proliferation of vascular smooth muscle cells and the effects of rosiglitazone on the proliferation. Results Rosiglitazone inhibits CXCL16 induced the proliferation of vascular smooth muscle. Conclusion Rosiglitazone can inhibit the proliferation of vascular smooth muscle CXCL16. [Keywords:] rosiglitazone; CXC chemokine ligand 16; vascular smooth muscle cells Studies have shown that CXC chemokine ligand 16 (CXC chemokine ligand 16, CXCL16) as a novel chemokine ligand, can promote the proliferation of arterial smooth muscle (1). Rosiglitazone (RSG) as a peroxidase proliferator-activated receptor (PPAR ) agonists inhibit vascular smooth muscle cells (2.) examined the effects of RSG on the CXCL16-induced proliferation of vascular smooth muscle cells to verify its role in vascular smooth muscle cell proliferation, and to further explore its mechanism. 1 Materials and methods 1.1 The material of adult rats, irrespective of gender (by the Experimental Animal Center of Jilin University). RPMI1640 medium (Gibco Company USA), fetal bovine serum (Hangzhou Evergreen Biological Engineering Materials Co., Ltd.), recombinant murine CXCL16 (U.S. R amp; D systems company ), LY294002 (USA BioVision Company), pyrrolidine dithio carbamate (PDTC) (Sigma, USA), rabbit anti-smooth muscle actin in rats @ (@ SMA) polyclonal antibody (Wuhan Boster Biological Engineering Company) , rabbit anti-rat CXCL16 polyclonal antibody (Santa Cruz U.S. company), diaminobenzidine (DAB) chromogenic kit (Beijing Zhongshan Biotechnology Co., Ltd.). RT PCR kit (MBI Fermentas Lithuania company). primer from Shanghai Sangon Technical Services Ltd synthesis. other reagents were analytical pure. 1.2 Methods 1.2.1 rat