Eucommia drug-containing serum-induced bone marrow mesenchymal stem cells in experimental study.docVIP

 PAGE \* MERGEFORMAT 14 Eucommia drug-containing serum-induced bone marrow mesenchymal stem cells in experimental study Of: Zeng Chun, Fan Yue-ray Robert J. Ronald, had intended to Rong, Li Xian Peng, Yi Chun Chi [Abstract] Objective To study the kidney Chinese Eucommia serum containing mesenchymal stem cells (BMSCs) proliferation and directed differentiation. Methods 4 to 5 106 on behalf of MSCs seeded in 9 96, 3 groups, each Section 8 hole; two 6 well plates, each plate 3 hole, 2 d after the medium was changed by adding induction medium. divided into control group, dexamethasone group, Eucommia group. control group containing 10% fetal bovine serum (FBS) The L-DMEM medium and cultured, dexamethasone group in the basic medium by adding inducer ( glycerophosphate containing 10mmol / L, dexamethasone 0.1 mol / L, vitamin C 50 mg / L), Eucommia group with Eucommia 10% serum containing the L-DMEM medium., respectively, from the induction medium plus 1 day of using the MTT measurement of cell number 96, every day a board; in 10 days out a 6-well done ALP staining; the first 20 days to do 6-well plates by alizarin red staining. Results Eucommia OD values with serum group showed a clear upward trend, while the control group and dexamethasone group had no significant upward trend; the first 10 days and dexamethasone group Eucommia group of ALP staining, the first 13 days of calcium from the visible white nodules, the first 20 days of alizarin red staining. Conclusion Eucommia containing serum can promote bone marrow mesenchymal stem cells into bone cells and stimulate cell proliferation. [Keywords:] Eucommia; bone marrow mesenchymal stem cells; osteoblasts Abstract: ObjectiveTo study the generation and directional differentiation of BMSCs induced by Duzhong (eucommia bark) which can invigorate the kidney. MethodsInoculated quartus generation MSCs in nine ninety six-hole cell culture plates and two six-holes cell culture plates by 5 106 cells per millilit