Experimental study on the toxicity of certain Xylooligosaccharides.docVIP

 PAGE \* MERGEFORMAT 10 Experimental study on the toxicity of certain Xylooligosaccharides Xylo-oligosaccharides in the oligosaccharide occupies an important position, first of its effective dose in a number of oligosaccharides is very low, on the proliferation of Bifidobacterium for its part, the effective intake of 0.7g / man-days, in order to Isomaltooligosaccharide 1 / 20, oligo-fructose 1 / 10, oligo-galactose 1 / 7; Xylooligosaccharides research and development in China has just emerged in recent years [2] We are one of the catharsis of xylo-oligosaccharides with Runchang films were toxicological safety evaluation. The results showed that in mice acute toxicity of the product as a non-toxic substance test, Ames test, mouse bone marrow polychromatic erythrocyte micronucleus test, mouse sperm abnormality test for non-genetic toxicity, rat 30-day feeding tests showed that all experimental growth and development of animals, and all targets were within the normal ranges in this room, and the control group, no significant difference between the nature of pathological examination is no exception. Shows that the sample of the observation in rats did not result in significant toxic effects of indicators, consumption of the product is safe and reliable. 1 Materials and methods 1.1 Materials 1.1.1 xylooligosaccharides tablets provided by a Bio-Technology Co., Ltd.. 1.1.2 Experimental Animals Kunming mice of clean grade and clean-level Wistar rats were from the PLA Academy of Military Medical Experimental Animal Center, qualified证号SCXK-(Army) 2002-001. 1.1.3 Ames test strains by the Shandong Academy of Medical Sciences Institute of Labor Health and occupational diseases. 1.2 Method [1] 1.2.1 Acute Toxicity Test selection in mice 20, 10 each male and female, weight 18 ~ 22g. Dose of 10g/kg.bw, fed subjects given material, by gavage volume 0.2ml/10g.bw. Observation of 14 days, recording the