Ghrelin on the THP1 derived foam cells ATP binding cassette transporter G1 and means the regulation of.docVIP

 PAGE \* MERGEFORMAT 16 Ghrelin on the THP1 derived foam cells ATP binding cassette transporter G1 and means the regulation of Of: Jing Jing Mei Chunli Wang Yanfu million into the Bei Liu Wei Ke Li He Ping [Abstract] Objective Ghrelin on the human monocyte cell line (THP 1) derived foam cells ATP binding cassette transporter G1 (ABCG1) expression in rats and its possible way. Methods Cultured THP 1, by phorbol ester (PMA ) function to differentiate into macrophages, and then in oxidized low density lipoprotein (ox LDL) under conditions of existence and further into foam cells, oil red O staining identified the formation of foam cells. using RT PCR method and were detected by Western blot Ghrelin and closed after the intervention of peroxisome proliferator-activated receptor (PPAR) after the ABCG1 mRNA and protein expression. using enzymatic, fluorescence spectrophotometer by cholesterol and cholesterol efflux rate. Results Ghrelin significantly reduced intracellular the formation of lipid droplets, increased cholesterol efflux, and significantly increased monocyte / macrophage foam process of ABCG1 mRNA and protein expression, this effect was concentration-dependent. closed after PPAR, Ghrelin effect of inhibiting the formation of foam cells was blocked off, and the higher the concentration of PPAR antagonists, which block the effect of the more obvious. Conclusion Ghrelin ABCG1 transcription and translation by upregulating the level of delay the occurrence of atherosclerosis, and this effect may play a role through the PPAR pathway. [Keywords:] Ghrelin, peroxisome proliferator-activated receptor , ATP binding cassette transporter G1, foam cells, atherosclerosis [Abstract] Objective To investigate the effects of Ghrelin on the expression of ATP binding cassette transporter G1 (ABCG1) in THP 1 derived foam cells and its possible mechanism. Methods Human monocytic leukemia cell line (THP 1) was chosen. The differentiation of THP 1 cells into macropha