HPLC method for the determination of hydrochloric acid HCl Ivy Ivy alkali content of external application of powder_0.docVIP

 PAGE \* MERGEFORMAT 9 HPLC method for the determination of hydrochloric acid HCl Ivy Ivy alkali content of external application of powder Of: GUO Yan, Li Min, Shi Lei, Li Yongji [Abstract] Objective To develop a topical powder in hydrochloric acid hydrochloric acid ivy ivy Determination of alkali content. Methods HPLC method, Diamonsil ODS C18 column (250 mm × 4.6 mm, 5 μm), mobile phase phosphate buffer (0.01 mol / L dipotassium hydrogen phosphate solution, plus 1 ‰ triethylamine, pH adjusted to 3.0 with phosphoric acid) - methanol (80:20), flow rate 1.0 mL / min, detection wavelength 265 nm. Results in 0.446 sinomenine ~ 4.46 μg good linear relationship, the regression equation: Y = 9.01 × 106X +102 803 (r = 0.999 9). The average recovery was 99.2%, RSD = 0.68% (n = 9). Conclusion The method is sensitive , accurate, reproducible and can be used as external application of hydrochloric acid powder ivy quality control. [Keywords:] hydrochloride powder topical ivy, sinomenine, HPLC, determination Abstract: Objective To establish a method for the determination of content in Sinomenine Hydrochloride external applied powder. Methods Diamonsil ODS C18 (250 mm × 4. 6 mm, 5 μm) column was used in HPLC with mobile phase of phosphate buffer (0.01 mol / L K2HPO4 solution, and 1 ‰ triethylamine, pH was adjusted to 3.0)-MeOH (80:20). The flow rate was 1.0 mL / min and the detection wavelength was 265 nm. Results The linear range of Sinomenine Hydrochloride was 0.446 ~ 4.46 μg and the regression equation was Y = 9.01 × 106X +102 803 (r = 0.999 9). The average recovery was 99.2%, RSD = 0.68% (n = 9). Conclusion This method is rapid and accurate with good reproducibility. It can be applied to control the quality of Sinomenine Hydrochloride external applied powder. Keywords:: Sinomenine Hydrochloride external applied powder, Sinomenine Hydrochloride, HPLC, content determination Sinomenine (sinomenine) is Caulis Sinomenium acutum (Thunb.) Rehd. Et Wils. Alkalo