Oxidative stress on endothelial cell NFB iNOS expression and NO signaling.doc

 PAGE \* MERGEFORMAT 13 Oxidative stress on endothelial cell NFB iNOS expression and NO signaling Of: HE JIANG WANG Yan-ping LIU Li Bin, Chen Min Liu Xiaoying [Abstract] Objective To observe the oxidant tertiary butyl peroxide (t BHP on cultured endothelial cell viability and nuclear factor B (NF B, iNOS and nitric oxide (NO. Methods Human umbilical vein endothelial cells (HUVEC, with different concentrations of t BHP treatment, as follows: (1 to detect cell survival. t BHP role in concentrations 12.5,25,50,100 mol / L, the role of time was 30,60,90,120 min, using MTT observed, (2 test NF B p65, iNOS protein expression and intracellular NO levels. t BHP’s concentration was 50 mol / L, the role of time was 30,60,90,120 min, measured by Western blot NF B p65, iNOS protein expression , Griess Huaxue The cell color France, NO ??, (3iNOS inhibitor L-nitro arginine methyl ester (L NAME, 600 mol / L pretreated endothelial cells 24 h, significant color method with Griess Huaxue Detection of intracellular NO levels. Results t BHP can reduce endothelial cell viability in a time and dose dependent, 50 mol / L t BHP role in the nucleus 30 min activation of NF B p65 protein fragments peaked 60 min cytoplasmic role of iNOS protein peak, iNOS inhibitor L NAME significantly inhibited endothelial cell NO increase. Conclusions Oxidative stress can cause endothelial cell injury, the effect may way through the NF B iNOS NO mediated. [Keywords:] tertiary butyl peroxide, umbilical vein endothelial cells, oxidative damage, nuclear factor B, inducible nitric oxide synthase, nitric oxide Effects of Oxidative Stress on NF B, iNOS and NO in Endothelial Cells HE Jiang, CHEN Min, LIU Xiaoying, WANG Yanping, LIU Libin Fujian Institute of Endocrinology, The Affiliated Union Hospital, Fujian Medical Universiy, Fuzhou 350001 ABSTRACT: Objective To explore the effects of Tert butyl hydroperoxide (t BHP) on NF B, iNOS and NO in human umbilical vein endothelial cells. Methods