Pentoxifylline on myocardial injury.doc

 PAGE \* MERGEFORMAT 11 Pentoxifylline on myocardial injury Study: Fan Zheng Zhi-Gang Xu Chen Wang Chunmei Wang Chunyan Song Yanfang Abstract Objective pentoxifylline (PTX) on myocardial injury and its mechanism. Methods of myocardial ischemia and reperfusion (I / R) model, using Langendorff perfusion apparatus. 120 Wistar rats were randomly divided into normal group, ischemic group, I / R 5,10,20,30 min group, the corresponding PTX (100 mol / L) treatment group, continuous observation of PTX effects on hemodynamics . ELISA assay of myocardial tissue tumor necrosis factor (TNF ) content. Results before ischemia and reperfusion of the PTX 100 mol / L significantly restored in rats administered I / R before and after the heart of the left ventricular developed pressure (P lt;0.05), left ventricular end-diastolic pressure also decreased significantly; 100 mol / L PTX treatment Group I / R 5 min significantly reduced when TNF levels (P lt;0.05). Conclusion PTX increased ischemic myocardial contractility after hypoxia recovery level, and increase tolerance to myocardial ischemia and hypoxia. Improve myocardial I / R induced TNF generation may be the one of the mechanisms of protective effects. Keywords: of pentoxifylline; ischemia-reperfusion; tumor necrosis factor Research shows that Pentoxifylline (pentoxifylline, PTX) has a protective cardiovascular damage in patients with chronic heart failure and idiopathic dilated cardiomyopathy patients with cardiac function 1, but its effect on myocardial ischemia and reperfusion (ischemia reperfusin, I / R) injury and its mechanism has not been reported. This paper aims to study PTX on myocardial injury and the mechanism for the clinical application of PTX to provide a theoretical basis. 1 Materials and methods 1.1 Materials and animals clean Wistar rats (Ji 2003 0001), weighing 250 ~ 300 g, from Jilin University School of Medicine Laboratory Animal Center; PTX were purchased from Sigma,