Purine nucleotides on heroin-treated C6 rat glioma cell proliferation.doc

 PAGE \* MERGEFORMAT 9 Purine nucleotides on heroin-treated C6 rat glioma cell proliferation Of: Li Hongmei Li Kun, HE Hai Liu Jiankai Hong Min [Abstract] Objective To investigate heroin and purine nucleotides in rat C6 glioma cells. Methods rat C6 glioma cells, respectively, with different concentrations of heroin and purine nucleotide treated cells, MTT colorimetric assay for heroin and purine nucleotides on cell proliferation. MTT test results indicate that heroin rat C6 glioma cells inhibited, 120 mg / L heroin effects on C6 cells 24 h inhibition rate of 52%, and in a dose-dependent, purine nucleotides on the proliferation of C6 cells could promote, 120 mg / L the role of purine nucleotides 24 h, cell proliferation rate of 30%, and in a dose dependent . Conclusion of heroin inhibit the proliferation of C6 glioma cells, purine nucleotides in rat C6 glioma cell proliferation, and to some extent against the heroin on C6 cell proliferation. [Keywords:] purine nucleotide, heroin, glioma cells, cell proliferation. [Abstract] Objective To investigate the effects of heroin and purine nucleotides on the proliferation of C6 glioma cells. Methods The effects of heroin and purine nucleotides on the proliferation of C6 glioma cells were evaluated by MTT assay. Results Heroin inhibited the growth of C6 glioma cells in vitro, 52% C6 glioma cells were inhibited when the cells were treated with 120 mg / L heroin for 24 h, purine nucleotides promoted the proliferation of C6 glioma cells, 30% C6 glioma cells were promoted when the cells were treated with 120 mg / L purine nucleotides for 24 h. Conclusions Heroin inhibits the proliferation of C6 glioma cells, purine nucleotides promotes the proliferation of C6 glioma cells. [Keywords:] Purine nucleotides, Heroin, Glioma cells, Proliferation Rat C6 glioma cell membranes as the existence of opioid receptors, has been widely used to study various mechanisms of opioid model system (1,2). The laboratory has found