Radix decoction of the genetic toxicity studies.docVIP

Radix decoction of the genetic toxicity studies Of: Hu Yanping, Song Jie, Wang Xin, Zhang Min, WANG Xiu-wen, Li Bo [Abstract] Objective mouse lymphoma assay (MLA) and mouse bone marrow micronucleus test (MNT) of the genotoxicity of water decoction of Radix. Methods in the MLA, 3.36,6.73,13.45,26.90 mg (crude drug ) / mL 4 different concentration groups in non-metabolic activation (-S9) and metabolic activation (+ S9) conditions, and L5178Y cells 3 h, the expression 2 d, prepared by mutation frequency plate and cultured 12 d, counting large and small colony hole number, calculate the total