Radix decoction of the genetic toxicity studies.doc

Radix decoction of the genetic toxicity studies Of: Hu Yanping, Song Jie, Wang Xin, Zhang Min, WANG Xiu-wen, Li Bo [Abstract] Objective mouse lymphoma assay (MLA) and mouse bone marrow micronucleus test (MNT) of the genotoxicity of water decoction of Radix. Methods in the MLA, 3.36,6.73,13.45,26.90 mg (crude drug ) / mL 4 different concentration groups in non-metabolic activation (-S9) and metabolic activation (+ S9) conditions, and L5178Y cells 3 h, the expression 2 d, prepared by mutation frequency plate and cultured 12 d, counting large and small colony hole number, calculate the total mutation rate (MF) and small colony mutant percentage (SC%), in the MNT in 10 ICR mice in each group, male and female, 13.35,6.68,3.34 g (crude drug) / kg 3 A dose interval of 24 h intragastric administration of 2, making bone marrow smears, counting each mouse 2 000 polychromatic erythrocytes containing micronuclei in polychromatic erythrocytes, calculating each animal micro polychromatic erythrocytes average nucleation rate. Results MLA4 a concentrations in the + /-S9 conditions induced a dose-related increase in MF, with the negative control group was statistically significant (P lt;0.01), SC% similar to the negative control group, MNT of each dose group did not show bone marrow suppression, induced micronuclei compared with the negative control group no significant increase. Conclusion Radix decoction in the + /-S9 under the conditions of L5178Y cells can be induced tk mutation, suggesting that it mutagenic substances may exist, but that the test on mouse bone marrow cell chromosome no damage, the metabolic activation in vivo genetic toxicity does not appear. [Keywords:] Radix decoction lymphoma assay, micronucleus test, genetic toxicity, mice Abstract: Objective To investigate genotoxicity of Radix Ophiopogonis decoction by Mouse Lymphoma Assay (MLA) and Mouse Bone Marrow Micronucleus Test (MNT). Methods In MLA, four dose levels of 3.36, 6.73, 13.45, 26.90 mg (crude