Radix trichosanthin from different areas Determination of citrulline.doc

 PAGE \* MERGEFORMAT 8 Radix trichosanthin from different areas Determination of citrulline Authors: Yang Yuqin, Li-Yan Zhang, Li Jian, Zhou Ping [Abstract] Objective To TLC Determination of trichosanthin from different areas in the citrulline content of the establishment of method for determination of citrulline. Methods start agent: n-butanol - ethanol - acetic acid - water (9.5:2:3.5:3); reagent: 1% ninhydrin test solution; detection wavelength: λ S = 510 nm, λ R = 700 nm . Results citrulline in 0.200 8 ~ 2.008 μ g, the peak area and showed a good linear relationship (r = 0.999); the average recovery was 99.12%, RSD = 1.12%. Conclusion The method is simple, rapid, accurate, and can be used for the determination of citrulline. [Keywords:] trichosanthin citrulline TLCS Abstract: ObjectiveTo develop an TLC method for the determination of trichosanthin Citrullin content from different habitats.MethodsButanol-ethanol-acetic acid-H2O (9.5:2:3.5:3) was used as the mobile phase, 1% ninhydrin test solution was used as reagent, the detection wavelength was λ S = 510 nm, λ R = 700 nm. ResultsThere was a good linear relationship within the range of 0.2008 ~ 2.008 μ g. The average recovery was 99.12%, RSD = 1.12%. ConclusionThe proposed method is simple, rapid and accurate.It can be used to determine the contents of Citrullin in Radix Trichosanthis .. Keywords:: Radix Trichosanthis.; Citrulline; TLC Cucurbitaceae Trichosanthes Trichosanthes for Trichosanthes KiriLowii Maxim. Or bilateral Trichosanthes Trichosanthes rosthornii Harms of dry root [1], slightly cold, sweet, slightly bitter flavor, owned by the lung, stomach. There is heat Sheng Jin, swelling removing the abscess, the effectiveness of anti-cancer. For fever, polydipsia, Hyperactivity dry cough, heat, etc. Diabetes Syndrome. TLC-scanning method used in this experiment [2] on the trichosanthin from different areas were determined to establish a method for determination citrulline. An instrument and re