Regulation of survivin promoter HSV1TK eukaryotic expression vector and A549 lung cancer cell lines on the killing effect.docVIP

 PAGE \* MERGEFORMAT 16 Regulation of survivin promoter HSV1TK eukaryotic expression vector and A549 lung cancer cell lines on the killing effect [Abstract] Objective: To construct survivin (survivin, SURV) promoter regulated herpes simplex virus type 1 thymidine kinase (HSV1 TK) gene expression vector, to detect the promoter activity and specificity of the gene observed in vivo in rats Growth suppression of lung cancer. Methods: Recovery SURV digested promoter, CMV promoter; PCR amplification of HSV1 TK gene; to the fragment containing the reporter gene luciferase gene (Luciferase, Luc) in the multiple cloning vector pGL3 Basic point connection, were constructed as SURV, CMV promoter control HSV1 TK, Luc eukaryotic expression vector (pSURV TK, pCMV TK, pSURV Luc, pCMV Luc), with the liposome vector transfected A549 cells, protein blot TK gene expression, experimental observation ganciclovir cytotoxic activity of TK protein, tumor growth inhibition experiments confirmed the inhibitory function of pSURV TK. Results: successfully SURV promoter, CMV promoter, HSV1 TK cloned into the reporter vector pGL3 multiple cloning sites, restriction enzyme digestion and DNA sequence analysis, Western blotting confirmed pSURV TK A549 lung cancer cells can be in the specific expression of the TK protein, ganciclovir cytotoxicity experiments showed that the expression of TK protein activity, can inhibit the growth of lung cancer in vivo. Conclusion: SURV TK luciferase gene therapy vector may be specific regulation of TK gene expression in A594 lung cancer cells and inhibit cancer cell growth, for the application of suicide gene therapy provides a new way of lung cancer. [Keywords:] lung cancer; gene therapy; survivin; promoter; herpes simplex virus type 1 thymidine kinase [Abstract] Objective: To construct a lung cancer specific the herpes simplex virus 1 thymidine kinase (HSV1 TK) expression vector regulated by survivin promoter (SURV) and detect its activation and po