RPHPLC determination of Naodesheng film HSYA A content of.doc

 PAGE \* MERGEFORMAT 10 RPHPLC determination of Naodesheng film HSYA A content of [Abstract] Objective RP-HPLC method established for the determination of Naodesheng film HSYA A concentration method. Ultrasonic Extraction Methods by purified HP20 macroporous resin, to RP-HPLC determination of HSYA A content. Column was Phenomenex Luna C18 (5 μ m, 3.9 mm * 150 mm), mobile phase of acetonitrile-methanol   0.7% phosphoric acid solution (volume ratio 21:5:74), flow rate of 1 .0 mL * min-1, column temperature 30 ℃ , detection wavelength 403 nm. Results HSYA A at 1.22 ~ 9.15 μ g / mL range a good linear relationship, a correlation coefficient of 0.9998. The average recovery was 100.93%, RSD 1.73%. Conclusion The method is accurate, specific, reproducible, and can be used for the determination of Naodesheng film HSYA A content. [Keywords:] Naodesheng Tablet HSYA A determination of macroporous resin Naodesheng films from 37, Chuanxiong, safflower, Kudzu, and hawthorn etc., with blood stasis, dredge meridians, Xingnao resuscitation of the effectiveness of [1]. The 2005 edition of ‘Chinese Pharmacopoeia’ only 37 saponins for quantitative indicators of Naodesheng Tablet quality control [1]. The safflower as an important part of the drug, the main active ingredient HSYA A (hydroxyl safflor yellow A, HSYA) with the expansion of coronary arteries, relieve myocardial ischemia and anti-coagulation, inhibition of thrombosis and so the role of [ 2, 3], in the treatment of Naodesheng Tablet cerebrovascular disease plays an important role. At present, on the film safflower Naodesheng quality control method of composition has not been reported. In this paper, the determination of the film HSYA Naodesheng content of RP  HPLC method, in order to further control the quality of Naodesheng tablets provide an experimental basis. An instrument and reagent 1.1 Instrument Waters 2695-type high-performance liquid chromatography; Waters 2996 diode array detector; Waters Empower