Circulating Levels of Human salusin-β,a Potent Hemodynamic and Atherogenesis Regulator.docVIP

Circulating Levels of Human salusin-β,a Potent Hemodynamic and Atherogenesis Regulator Kazumi Fujimoto , Akinori Hayashi , Yuji Kamata , Akifumi Ogawa , Takuya Watanabe , Raishi 1,2 2 2 2 3 Ichikawa , Yoshitaka Iso , Shinji Koba , Youichi Kobayashi , Takatoshi Koyama , Masayoshi Shichiri 2 4 4 4 1 2* 1 Laboratory of Molecular Genetics of Hematology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan, 2 Department of Endocrinology, Diabetes and Metabolism, Kitasato University School of Medicine, Kanagawa, Japan, 3 Laboratory of Cardiovascular Medicine, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan, 4 Division of Cardiology, Department of Medicine, Showa University School of Medicine, Tokyo, Japan Abstract Using bioinformatics analysis, we previously identified salusin-β, an endogenous bioactive peptide with diverse physiological activities. Salusin-β is abundantly expressed in the neuroendocrine system and in systemic endocrine cells/macrophages. Salusin-β acutely regulates hemodynamics and chronically induces atherosclerosis, but its unique physicochemical characteristics to tightly adhere to all types of plastic and glassware have prevented elucidation of its precise pathophysiological role. To quantitate plasma total salusin-β concentrations, we produced rabbit and chicken polyclonal antibodies against the C- and N-terminal end sequences, circumvented its sticky nature, and successfully established a sa