山东百部遗传多样性分析.docVIP

Botanical Research植物学研究, 2013, 2, 1-5 /10.12677/br.2013.21001 Published Online January 2013 (/journal/br.html) Genetic Diversity of Stemona shandongensis Populations* Lei Zhang, Yan Ma, Dekui Zang # Forestry College, Shandong Agricultural University, Taian # Email: zangdk@ Received: Sep. 7 , 2012; revised: Sep. 15 , 2012; accepted: Sep. 28 , 2012 th th th Abstract: Genetic diversity and genetic structure of 120 individuals of four Stemona shandongensis popula- tions were investigated by AFLP method. A total of 282 bands were observed with 8 primer combination, in- cluding 137 polymorphic bands. The percentage of polymorphic bands was 48.58%. In species level, Nei’s gene diversity index was 0.1441; Shannon’s information index was 0.2485; total gene diversity was 0.1646; population gene diversity was 0.1682, gene differentiation coefficient was 0.0210. UPGMA cluster based on AFLP makers was similar to the cluster based on morphological traits; there was no significant correlation between genetic distance and geographic distance. The results showed that there was genetic differentiation among populations, and it could express by morphological characteristics. Keywords: AFLP; Stemona shandongensis; Genetic Diversity; Population 山东百部遗传多样性分析* 张 雷，马 燕，臧德奎# 山东农业大学林学院，泰安 Email: zangdk@ # 收稿日期：2012年 9月 7日；修回日期：2012年 9月 15日；录用日期：2012年 9月 28日 摘 要：利用 AFLP分子标记技术对山东百部 4个居群 120个样品进行了遗传多样性分析。8对引物 组合共扩增出 282条清晰可辨条带，其中多态性条带 137条，多态性位点百分率 48.58%；在种级水平 上，Nei’s基因多样性指数为 0.1441，Shannon多态性信息指数为 0.2485；居群总遗传多样性(H )为 t 0.1646，居群内遗传多样性为 0.1682，基因分化系数为 0.0210。UPGMA聚类表明，AFLP聚类结果与 表型分化聚类结果一致，居群间具有一定程度的遗传分化，并通过表型性状表现出来。 关键词：AFLP；山东百部；遗传多样性；居群 1.引言 [4-10] 的遗传结构和遗传多样性分析 。本研究拟采用 AFLP分子标记，以居群为单位，通过分析山东百部 的遗传多样性水平和遗传结构，探讨影响其遗传多样 性和遗传结构的因素，分析濒危原因，并提出保护策 略。 山东百部(Stemona shandongensis )为百部科百部 属植物，与直立百部 (S. sessilifolia )和蔓