C_57_BL_6J小鼠主要器官的组织学数据库的建立.pdfVIP

# 166# 局解手术学杂志 20 10 19 3 J Regional Anat Operative Surg, 2010, V ol. 19, N o. 3 C BL/6J 57 1 2 3a 3b 3b , , , , ( 1. , 40071 ; 2. 324, 400020; 3. : a. ; b. , 400038) [ ] C BL /6J C BL /6J 10, 10, 7 7 , 4 , 10% , , HE , , , SQLSEVER2000, C#, B/S( /) 20 C BL /6J, 1 89, 13 78, 1 63, 7 7. 8 GB C BL /6J C BL /6J, 7 7 [ ] C BL /6J; ; ; 7 [ ] Q9 4. 6; C37 [] A [] 1672- 042(2010) 03-0166-02 Establishing a digitalhistology library of themain organ of theC BL/6Jmice 7 DENG Yu-jiang, CHEN Jun-ying, Q IU Quan-guang, WANG Yong, WEIH ong ( L ife Science Institute, Sou thwest Un ivers-i ty, Chongqing 40071 , Ch ina) Abstract: Objective To estab lish a d igital histology library of the m ain organ of the C BL /6J m ice. ethods Twenty C BL /6J 7 7 m ice (m ale 10, fem ale 10) w ere anesthetized deep ly, totally 4 organs and tissuesw ere taken and fixed by 10% form alin, includ ing heart, liver, spleen, k idney, stom ach, etc. embedded by paraffin and sectioned, stained w ith haematoxylin and eos in, and observation underm -i croscope. Then the mi agesw erem arked in com puter. The h istology library w as established by SQLWEVER2000 databasem anagement sys- tem, C# programm ing language and Brow ser/Server. Results The m ain organs and tissuesw ere d issected, 1 89 pieces of sections w ere got, 13 78 mi agesw ere observed, and 1 63 p icturesw ere marked. A large histology library ( 7. 8 G )