原子力显微镜对不同方式处理的副流感病毒成像的研究_唐蕊华.pdf

27　 1 Vol.27　No.1 2010 　2 　　　　　　　 　　　　　Journal of Biomedical Engineering　　 　　　　　　　　　　February　2010 1 1Δ 2 1 1 1 1 唐蕊华 　薛小平 　尹　文 　苏　婧 　谢玉为 　尹焕才 　杨　慧 1(西北工业大学 生命科学院, 西安710072) 2(第四军医大学 中心实验室, 西安710032) 　:(atom force microscope, AFM)(para influenza virus, PIV), 。(transmission electron micro- scope, TEM)。 AFM , , , AFM 。T ritonx-100 , , , PIV 。S S , RNA, AFM , RNA 。AFM PIV 。 :;;Tritonx-100;S S 　Q939.4　　　A 　　　1001-5515(2010)01-0053-05 An Atomic Force Microscopy Study on t e Images of Para Influenza Virus under Different Treatment Conditions 1 1 2 1 1 1 1 Tang Rui ua 　Xue Xiaoping 　Yin Wen 　Su Jing 　Xie Yuwei 　Yin Huancai 　Yang Hui 1 (Col leg e of L if e S ciences , N ort hw estern P oly tech nic Univ ersi ty , X i'an 710072, China) 2 (The Cent ral L abor atory , F our th Mi l it ary Medi cal Uni v ersi ty , X i'an 710032, Chi na) Abstract:Using atomic force microscope (AFM), we investigated the images of Para influenza virus (PIV)under dif- ferent treatment conditions and observed the different appearances of the virus and its ultra-microstructure from the exterior to the interior.From the 2 images under transmission electron microscope (TEM ), we could see that the surfaces of PIV particles exhibited spherical and band-shaped`tufts';from the 3 images under AFM, we could fur- ther observe the whole spherical virus particles and their detailed surfaces, which exhibited round and band-shaped `tufts'.Comparing the images under T EM with those under AFM, we found that the latter could reveal the surface topogr