Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection 外文参考文献.docVIP

Short Communication Mediators of Inflammation, 10, 273–277 (2001) A RAPID, simple and low-cost assay method of hista- Rapid and simple determination of histamine-N-methyl transferase activity by high-performance liquid chromatography with UV detection mine-N-methyltransferase activity was developed. Methylhistamine, which was separated from the enzymatic reaction system on reversed-phase high- performance liquid chromatography using an ion- paired chromatographic technique, was detected spectrophotometrically at 226nm. The mobile phase used for the separation of methylhistamine was