A Novel Gene Signature for Molecular Diagnosis of Human Prostate Cancer by RT-qPCR 英文参考文献.docVIP

下载本文档

7
0
约5万字
约 9页
2017-05-11 发布于上海
举报
版权申诉

A Novel Gene Signature for Molecular Diagnosis of Human Prostate Cancer by RT-qPCR 英文参考文献.doc

1、本文档共9页，可阅读全部内容。
2、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。
3、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
5、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
6、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
7、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
8、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

A Novel Gene Signature for Molecular Diagnosis of Human Prostate Cancer by RT-qPCR 英文参考文献

ANovelGeneSignatureforMolecularDiagnosisof HumanProstateCancerbyRT-qPCR FedericaRizzi1,2.,LuciaBelloni1,2.,PellegrinoCrafa3,MircaLazzaretti3,DanielRemondini4 ,Stefania Ferretti5,PieroCortellini5,ArnaldoCorti6,SaverioBettuzzi1,2* 1DepartmentofMedicinaSperimentale,UniversityofParma,Parma,Italy,2IstitutoNazionaleBiostruttureeBiosistemi(I.N.B.B.),Roma,Italy,3DepartmentofPatologiae Medicinadilaboratorio,UniversityofParma,Parma,Italy,4Dimorfipa,UniversityofBologna,Bologna,Italy,5UrologyOperativeUnit,AziendaOspedaliera-Universitariaof Parma,Parma,Italy,6DepartmentofScienzeBiomediche,UniversityofModena,Modena,Italy Abstract Background: Prostate cancer (CaP) is one of the most relevant causes of cancer death in Western Countries. Although detectionofCaPatearlycurablestageishighlydesirable,actualscreeningmethodspresentlimitationsandnewmolecular approachesareneeded.GeneexpressionanalysisincreasesourknowledgeaboutthebiologyofCaPandmayrendernovel molecular tools, but the identification of accurate biomarkers for reliable molecular diagnosis is a real challenge. We describeherethediagnosticpowerofanovel8-genessignature:ornithinedecarboxylase(ODC),ornithinedecarboxylase antizyme(OAZ),adenosylmethioninedecarboxylase(AdoMetDC),spermidine/spermineN(1)-acetyltransferase(SSAT ),histone H3 (H3), growth arrest specific gene (GAS1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Clusterin (CLU ) in tumourdetection/classificationofhumanCaP. Methodology/PrincipalFindings:The8-genesignaturewasdetectedbyretrotranscriptionreal-timequantitativePCR(RT- qPCR)infrozenprostatesurgicalspecimensobtainedfrom41patientsdiagnosedwithCaPandrecommendedtoundergo radical prostatectomy (RP). No therapy was given to patients at any time before RP. The bio-bank used for the study consistedof66specimens:44werebenign-CaPpairedfromthesamepatient.Thirty-fivewereclassifiedasbenignand31as CaP after final pathological examination. Only molecular data were used for classification of specimens. The Nearest Neigh