ProtocolforTissueLysisforReversePhaseProteinArray.docxVIP

RPPA CORE | BCM | CPRIT2016 Protocol for Tissue Lysis for Reverse Phase Protein Array Reagents provided by Proteomics Core: 3ml RPPA Lysis Buffer (store at 4?C) 1ml 5X Protease Inhibitors (store at -20?C) 1ml 5X Phosphatase Inhibitors (store at -20?C) Choose one of the following sample buffer based on your estimated protein concentration: 1 ml 2x SDS Sample Buffer (store at 4?C) 0.5 ml 4x SDS Sample Buffer (store at 4?C) Reagents/supplies required, but not supplied by Proteomics Core 2-mercaptoethanol Sample Tubes RB (Qiagen, Cat# 990381) for tissue aliquot 1.5ml microcentrifuge tube wit