G-Quadruplex DNA Sequences Are Evolutionarily Conserved and Associated with Distinct Genomic Features in Saccharomyces cerevisiae 英文参考文献.docVIP

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G-Quadruplex DNA Sequences Are Evolutionarily Conserved and Associated with Distinct Genomic Features in Saccharomyces cerevisiae 英文参考文献.doc

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G-Quadruplex DNA Sequences Are Evolutionarily Conserved and Associated with Distinct Genomic Features in Saccharomyces cerevisiae 英文参考文献

G-QuadruplexDNASequencesAreEvolutionarily ConservedandAssociatedwithDistinctGenomic FeaturesinSaccharomycescerevisiae JohnA.Capra1,KatrinPaeschke2,MonaSingh1*,VirginiaA.Zakian2* 1DepartmentofComputerScience,Lewis-SiglerInstituteforIntegrativeGenomics,PrincetonUniversity,Princeton,NewJersey,UnitedStatesofAmerica,2Department ofMolecularBiology,PrincetonUniversity,Princeton,NewJersey,UnitedStatesofAmerica Abstract G-quadruplexDNAisafour-strandedDNAstructureformedbynon-Watson-Crickbasepairingbetweenstackedsetsoffour guanines. Many possible functions have been proposed for this structure, but its in vivo role in the cell is still largely unresolved.Wecarriedoutagenome-widesurveyoftheevolutionaryconservationofregionswiththepotentialtoformG- quadruplexDNAstructures(G4DNAmotifs)acrosssevenyeastspecies.WefoundthatG4DNAmotifsweresignificantly more conserved than expected by chance, and the nucleotide-level conservation patterns suggested that the motif conservationwastheresultoftheformationofG4DNAstructures.Wecharacterizedtheassociationofconservedandnon- conservedG4DNAmotifsinSaccharomycescerevisiaewithmorethan40knowngenomefeaturesandgeneclasses.Our comprehensive,integratedevolutionaryandfunctionalanalysisconfirmedthepreviouslyobservedassociationsofG4DNA motifswithpromoterregionsandtherDNA,anditidentifiedseveralpreviouslyunrecognizedassociationsofG4DNAmotifs withgenomicfeatures,suchasmitoticandmeioticdouble-strandbreaksites(DSBs).ConservedG4DNAmotifsmaintained strongassociationswithpromotersandtherDNA,butnotwithDSBs.WealsoperformedthefirstanalysisofG4DNAmotifs inthemitochondria,andsurprisinglyfoundatenfoldhigherconcentrationofthemotifsintheAT-richyeastmitochondrial DNA thanin nuclear DNA. The evolutionary conservationof the G4 DNA motifand its association with specific genome featuressupportsthehypothesisthatG4DNAhasinvivofunctionsthatareunderevolutionaryconstraint. Citation:CapraJA,PaeschkeK,SinghM,ZakianVA(2010)G-QuadruplexDNASequencesAreEvolutionarilyConservedandAssociat