Mechanistic Basis of Branch-Site Selection in Filamentous Bacteria 英文参考文献.docVIP

下载本文档

0
0
约4.76万字
约 8页
2017-05-12 发布于上海
举报
版权申诉

Mechanistic Basis of Branch-Site Selection in Filamentous Bacteria 英文参考文献.doc

1、原创力文档（book118）网站文档一经付费（服务费），不意味着购买了该文档的版权，仅供个人/单位学习、研究之用，不得用于商业用途，未经授权，严禁复制、发行、汇编、翻译或者网络传播等，侵权必究。。
2、本站所有内容均由合作方或网友上传，本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺！文档内容仅供研究参考，付费前请自行鉴别。如您付费，意味着您自己接受本站规则且自行承担风险，本站不退款、不进行额外附加服务；查看《如何避免下载的几个坑》。如果您已付费下载过本站文档，您可以点击这里二次下载。
3、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等，请点击“版权申诉”（推荐），也可以打举报电话：400-050-0827(电话支持时间：9:00-18:30)。
4、该文档为VIP文档，如果想要下载，成为VIP会员后，下载免费。
5、成为VIP后，下载本文档将扣除1次下载权益。下载后，不支持退款、换文档。如有疑问请联系我们。
6、成为VIP后，您将拥有八大权益，权益包括：VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
7、VIP文档为合作方或网友上传，每下载1次，网站将根据用户上传文档的质量评分、类型等，对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档

Mechanistic Basis of Branch-Site Selection in Filamentous Bacteria 英文参考文献

MechanisticBasisofBranch-SiteSelectionin FilamentousBacteria DavidM.Richards1.,AntjeM.Hempel1,2.,KlasFla¨rdh2*,MarkJ.Buttner1,MartinHoward1* 1JohnInnesCentre,NorwichResearchPark,Norwich,UnitedKingdom,2DepartmentofBiology,LundUniversity,Lund,Sweden Abstract Manyfilamentousorganisms,suchasfungi,growbytip-extensionandbyformingnewbranchesbehindthetips.Asimilar growth mode occurs in filamentous bacteria, including the genus Streptomyces, although here our mechanistic understanding has been very limited. The Streptomyces protein DivIVA is a critical determinant of hyphal growth and localizes in foci at hyphal tips and sites of future branch development. However, how such foci form was previously unknown.Here,weshowexperimentallythatDivIVAfocus-formationinvolvesanovelmechanisminwhichnewDivIVAfoci break off from existing tip-foci, bypassing the need for initial nucleation or de novo branch-site selection. We develop a mathematicalmodelforDivIVA-dependentgrowthandbranching,involvingDivIVAfocus-formationbytip-focussplitting, focus growth, and the initiation of new branches at a critical focus size. We quantitatively fit our model to the experimentally-measuredtip-to-branchandbranch-to-branchlengthdistributions.Themodelpredictsaparticularbimodal tip-to-branch distribution results from tip-focus splitting, a prediction we confirm experimentally. Our work provides mechanisticunderstandingofanovelmodeofhyphalgrowthregulationthatmaybewidelyemployed. Citation: RichardsDM,HempelAM,Fla¨rdh K,Buttner MJ,HowardM(2012)MechanisticBasisofBranch-SiteSelection inFilamentous Bacteria.PLoSComput Biol8(3):e1002423.doi:10.1371/journal.pcbi.1002423 Editor:ChristopherV.Rao,UniversityofIllinoisatUrbana-Champaign,UnitedStatesofAmerica ReceivedNovember28,2011;AcceptedJanuary26,2012;PublishedMarch8,2012 Copyright: ? 2012 Richards et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricteduse,distribution,andreprodu