单抗纯化柱说明书.pdfVIP

GE Healthcare Instructions 71-7002-00 AP HiTrap affinity columns HiTrap Protein A HP, 1 ml and 5 ml HiTrap™ Protein A HP is a prepacked ready to use, column for preparative purification of monoclonal and polyclonal antibodies. The special design of the column, together with the medium provide fast, simple and easy separations in a convenient format. The column can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTA™ design. Code No. Product No. supplied 17-0402-01 HiTrap Protein A HP 5 × 1 ml 17-0402-03 HiTrap Protein A HP 2 × 1 ml 17-0403-01 HiTrap Protein A HP 1 × 5 ml 17-0403-03 HiTrap Protein A HP 5 × 5 ml Connector kit Connectors Usage No. supplied supplied 1/16” male/luer Connection of syringe to top of HiTrap 1 female column Tubing connector Connection of tubing (e.g. Peristaltic 1 flangeless/ Pump P1) to bottom of HiTrap column1 M6 female Tubing connector Connection of tubing (e.g. Peristaltic 1 flangeless/M6 male Pump P1) to top of HiTrap column2 Union 1/16” Connection to original FPLC™ System 1 female/M6 male through bottom of HiTrap column Union M6 female/ Connection to original FPLC System 1 1/16” male through top of HiTrap column Stop plug female, S