显齿蛇葡萄叶对食品防腐作用的研究_刘胜贵要点.doc

Science and Technology of Food Industry 研究与探讨  显齿蛇葡萄叶对食品防腐作用的研究 刘胜贵，张 静，王频艺，付 明 （怀化学院生命科学系，民族药作植物资源研究与利用湖南省重点实验室，湖南怀化 418008） 摘 要：为了研究显齿蛇葡萄对食品的防腐效果，分别采摘3、7、11月份的显齿蛇葡萄叶作为实验材料，以大肠杆菌、金黄色葡萄球菌、苏云金芽胞杆菌、藤黄八叠球菌和枯草芽胞杆菌五种微生物为实验菌株，用药敏纸片扩散法测定不同生长时期的显齿蛇葡萄叶抑菌圈直径，通过二倍稀释法测定MBC值，以平板菌落计数反映添加有不同提取物浓度的食品中微生物的生长状况。结果表明7月份采摘的显齿蛇葡萄提取物对金黄色葡萄球菌抑菌作用最强，抑菌圈直径达到27.0mm，MBC为5%；当提取物浓度为4MBC时，肉汤食品抑菌时间延长达140h。显齿蛇葡萄有广谱抑菌作用，并且对猪肉、牛奶、西瓜和豆浆都有防腐作用。 关键词：显齿蛇葡萄，水提取物，药敏纸片扩散法，二倍稀释法，平板菌落计数法 Application on food preservation of Ampelopsis grossedentata leaves LIU Sheng-gui，ZHANG Jing，WANG Pin-yi，FU Ming （The Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of HUnan Province， Department of Life Sciences，Huaihua University，Huaihua 418008，China） Abstract：To research the application on food preservation of extract of Ampelopsis grossedntata，the leaves of A.grossedentata that were collected in March，July and November were taken as experimental materials， respectively. Escheridhia coli，Staphylococcus aureus，Bacillus thuringiensis，Sarcina lutea and Bacillus subtilis were taken as experimental strains. After the different bacteria were dealed with leaves extracts of A. grmsedntara ， the inhibitory zone diameters were evaluated by using a disk - diffusion and the minimum bactericidal concentrations （MBC） were detected by double dilution method during the process of bacterial growth. Microorganisms in the food solution，which were related to different concentrations of the extracts，were quantified by colony counting method. The results showed the extracts from the leaves of A.grossedentata were collected in July had the strongestantibacterial effect on S. aureus，the inhibitory zone diameter reached 27.0mm and MBC was only 5%. Bacteriostatic time for broth food could reach 140h when the concentration of the extracts was 4MBC. This indicated the extracts from the leaves of A.grossedentata had broad antimicrobial spectrum and good preservative effect on pork，milk，watermelon and soy milk. Key word