Single Molecule Fluorescence Image Patterns Linked to Dipole Orientation and Axial Position Application to Myosin Cross-Bridges in Muscle Fibers.PDF

Single Molecule Fluorescence Image Patterns Linked to Dipole Orientation and Axial Position: Application to Myosin Cross-Bridges in Muscle Fibers Thomas P. Burghardt* Department of Biochemistry and Molecular Biology and Department of Physiology and Biomedical Engineering, Mayo Clinic Rochester, Rochester, Minnesota, United States of America Abstract Background: Photoactivatable fluorescent probes developed specifically for single molecule detection extend advantages of single molecule imaging to high probe density regions of cells and tissues. They per