异养硝化-好氧反硝化菌株的筛选及初步鉴定.pdfVIP

河南农业科学.2014.43(3):59-64 Journal of Henan Agricultural Sciences 异养硝化-好氧反硝化菌株的筛选及初步鉴定 陈香琪，李 科，张利平铸 (河北大学生命科学学院/河北省微生物多样性研究与应用实验室，河北保定 071002) 摘要z 为了获得高效的异养硝化-好氧反硝化菌株，采用稀释涂布和发酵检测的方法从土壤和污水 中对其进行筛选，并通过分子生物学和形态观察方法对其进行初步鉴定。结果表明，从污水和土壤 中成功分离筛选到 SND104 、 SND305 、 SND2053 株同时具有异养硝化、好氧反硝化功能的菌株。 对筛选菌株进行异养硝化和好氧反硝化功能的研究表明，异养硝化培养过程中菌株对培养基中缤 态氮(NHt - N) 的去除率均达到 80% 以上，好氧反硝化培养过程中菌株对培养基中硝态氮(N(X - N) 的去除率均达到 50% 以上，并且在以上 2 个过程中总氮(TN) 和化学需氧量(COD) 的含量均降低 50% 以上。通过鉴定.SND104 、 SND305 属于假单胞菌属(Pseudomonas) .SND205 属于戴尔福特 菌属(Delftia) 。 关键词:异氧稍化;好氧反硝化;脱氮;筛选;鉴定 中固分类号: X172 文献标志码:A 文章编号: 1004 - 3268(2014)03 - 0059 - 06 Screening and Identification of Simultaneous Nitrification and Denitrification Bacteria CHEN Xiang-qi. Ll Ke.ZHANG Li-ping* (Key Lab of Microbial Diversity Research and Application of Hebei Province/ College of Life Sciences , Hebei University ,Baoding 071002 ,China) Abstract: In this article the methods of diluted coating and fermentation detection were adopted to achieve strains with high efficiency of heterotrophic nitrification and aerobic denitrification from soil and sewage. In the mean time preliminary appraisals were made using methods from molecular biology and morphology. Results indicated that 3 strains. SND104. SND305. SND205. which were successfully achieved from soil and sewage. possessed both heterotrophic nitrification and aerobic denitrification properties. The heterotrophic nitrification and aerobic denitrification function study of the screened strains showed that the removal rate of ammonium nitrogen(NHt - N) at hetero- trophic nitrification cultivation process exceeded 80 % and the removal rate