Ⅱ型胶原修饰脱细胞真皮基质胶原膜修复关节软骨缺损.docVIP

Ⅱ型胶原修饰脱细胞真皮基质胶原膜修复关节软骨缺损 张柏青1，张仲文2，孙 磊3，江 健3，吴 瑕4 (1武警北京市总队第三医院，北京市 100141；2武警总医院，北京市 100039；3北京积水潭医院，北京市 100035；4中国质量认证中心，北京市 100070) 引用本文：张柏青，张仲文，孙磊，江健，吴瑕. Ⅱ型胶原修饰脱细胞真皮基质胶原膜修复关节软骨缺损[J].中国组织工程研究，2017，21():871-876. DOI:10.3969/j.issn.2095-4344.2017.06.009 ORCID: 0000-0001-8561-0198(张柏青) 文章快速阅读： 文题释义： 脱细胞真皮基质胶原膜：Ⅱ型胶原修饰脱细胞真皮基质胶原膜：Collagen type II-modified acellular dermal membrane promotes the repair of cartilage defects Zhang Bai-qing1, Zhang Zhong-wen2, Sun Lei3, Jiang Jian3, Wu Xia4 (1Third Hospital of Beijing Armed Police Corps, Beijing 100141, China; 2General Hospital of Chinese Armed Police Corps, Beijing 100039, China; 3Beijing Jishuitan Hospital, Beijing 100035, China; 4China Quality Certification Center, Beijing 100070, China) Abstract BACKGROUND: Previous study has observed that the calf acellular dermal membrane exhibits slow repair efficiency, fast degradability speed and other shortcomings in the repair of cartilage defects. OBJECTIVE: To investigate the repair effect of the collagen type II-modified acellular dermal membrane on cartilage defects in rabbits. METHODS: The fetal rabbit chondrocytes were seeded onto the collagen type II-modified acellular dermal membrane, and the composite was then observed under scanning electron microscope at 3, 7 and 14 days. Cartilage defect models were established on the bilateral femoral condyles of 24 New Zealand white rabbits, and these model rabbits were randomly allocated to three groups. The cartilage-acellular dermal membrane and cartilage-collagen type II-modified acellular dermal membrane were implanted into the defect regions of control and experimental groups, respectively. Those received no intervention were as blank control group. Collagen type II immunohistochemical staining and Wakitani scoring system were performed at 6 and 12 weeks postoperatively. RESULTS AND CONCLUSION: Chondrocytes grew and adhered well in the scaffold. The Wakitani scores in the experimental group were significantly