Estimation of relatedness among non-pedigreed Yakutian cryo-bank bulls using molecular data implications for conservation and breed management.docVIP

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Estimation of relatedness among non-pedigreed Yakutian cryo-bank bulls using molecular data implications for conservation and breed management.doc

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Estimation of relatedness among non-pedigreed Yakutian cryo-bank bulls using molecular data implications for conservation and breed management

Tapio et al. Genetics Selection Evolution 2010, 42:28 /content/42/1/28 Genetics Selection Evolution RESEARCH Open Access Estimation of relatedness among non-pedigreed Research Yakutian cryo-bank bulls using molecular data: implications for conservation and breed management Ilma Tapio1, Miika Tapio1, Meng-Hua Li1, Ruslan Popov2, Zoya Ivanova2 and Juha Kantanen*1 Abstract Background: Yakutian cattle, the last remaining native cattle breed in Siberia, are well adapted to the extreme sub- arctic conditions. Nowadays only ca. 1200 purebred animals are left in Yakutia. The semen of six Yakutian bulls was stored in a cryo-bank without any pedigree documentation because of the traditional free herding style of the population. Methods: To clarify the genetic relatedness between these bulls and to provide recommendations to use their semen in future conservation and breed management programs, we have analysed 30 autosomal microsatellites and mitochondrial DNA sequences in 60 individuals including the six for which semen has been stored. Four relatedness estimators were calculated. In addition, we assessed the value of the cryo-bank bulls for the preservation of genetic variation of the contemporary Yakutian cattle by calculating allelic and gene diversity estimates and mean molecular coancestries. Results: On the basis of microsatellite variability, including the Yakutian cryo-bank bulls increases the allelic variation in the contemporary population by 3% and in the male subpopulation by 13%. In terms of the mean molecular coancestries, they are less related to the contemporary cow population than the breeding bulls and therefore could be used to reduce inbreeding in the living population. Although 30 loci are insufficient to resolve definitely their relatedness categories, the data suggest four pairs of cryo-bank bulls as possible half-sibs. Conclusions: Our results show that even relatively limited cryo-bank storage of semen can carry allelic variation thro