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Evaluation of a fiberoptic-based system for measurement of optical properties in highly attenuating turbid media.doc

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Evaluation of a fiberoptic-based system for measurement of optical properties in highly attenuating turbid media

BioMedical Engineering OnLine BioMedCentral Research Open Access Evaluation of a fiberoptic-based system for measurement of optical properties in highly attenuating turbid media Divyesh Sharma, Anant Agrawal, L Stephanie Matchette and T Joshua Pfefer* Address: Food and Drug Administration, Center for Devices and Radiological Health, Rockville, Maryland, USA Email: Divyesh Sharma - divyesh_sharma2001@; Anant Agrawal - anant.agrawal@; L Stephanie Matchette - stephanie.matchette@; T Joshua Pfefer* - joshua.pfefer@ * Corresponding author Published: 23 August 2006 Received: 04 April 2006 Accepted: 23 August 2006 BioMedical Engineering OnLine 2006, 5:49 doi:10.1186/1475-925X-5-49 This article is available from: /content/5/1/49 ? 2006 Sharma et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Abstract Background: Accurate measurements of the optical properties of biological tissue in the ultraviolet A and short visible wavelengths are needed to achieve a quantitative understanding of novel optical diagnostic devices. Currently, there is minimal information on optical property measurement approaches that are appropriate for in vivo measurements in highly absorbing and scattering tissues. We describe a novel fiberoptic-based reflectance system for measurement of optical properties in highly attenuating turbid media and provide an extensive in vitro evaluation of its accuracy. The influence of collecting reflectance at the illumination fiber on estimation accuracy is also investigated. Methods: A neural network algorithm and reflectance distributions from Monte Carlo simulations were used to generate predictive models based on the two geometries. Absolute measurements of diffuse reflectance were e