1308062115贾洋鸡肝DNA的提取与定量精讲.docVIP

摘 要 随着科学技术的不断进步，人们越来越多的了解到生物工程对人类社会的巨大帮助，特别是基因工程。通过对生物体内DNA的提取与分析，充分的了解生物繁衍的奥秘，也通过对DNA的研究研发出许多可以用于医学治疗药物和疾病预防的方法，提高了人类的生活质量。鉴于前面学者们对DNA的研究，设计出了许多关于DNA提取的方法，而且都已相对比较成熟。文章以咸阳市咸阳师范学院附近的鸡为研究对象，通过盐析法对鸡的肝脏中的DNA进行提取，然后再通过紫外可见分光光度法和定磷法法对DNA进行定量分析，最终确定所提取的DNA的含量。通过称量共从9g鸡肝中提取出0.532gDNA粗提物，通过两种定量分析方法对粗提物进行定量分析可知提取率并不是很高，用紫外可见分光光度法测得的粗提DNA溶液浓度大约为22.95μg/ml，可算出DNA的含量为0.0013g/g；用定磷法测得DNA的含量为0.0011g/g，说明两种检测方法的精确度有差异，但不是很明显。通过与其他研究人员的实验数据作对比可知蛋白质去除不是很理想。 关键词：Abstract With the progress of science and technology, more and more people understand that bio-engineering human society a great help, especially genetic engineering. Through the in vivo DNA extraction and analysis, a full understanding of the mysteries of biological reproduction, but also through the study of DNA is developed many ways for medical treatment and disease prevention, improve the quality of human life. Given the previous research on the DNA of scholars, we devised a number of methods on DNA extraction, and are relatively mature. Articles in Xianyang City, Xianyang Normal University nearby chicken for the study, by salting of chicken liver DNA was extracted, and then by UV-visible spectrophotometry and phosphorus measurement method for quantitative analysis of DNA, and ultimately determine the the content of the extracted DNA. Weighing apparent total chicken liver extract from 9g out 0.532g crude extracts, two quantitative analysis of crude extract quantitative analysis shows that the extraction rate is not very high, with a UV-visible spectrophotometry measured crude extract DNA concentration of about 22.95μg / ml, can be calculated DNA content of 0.0013g / g; as measured by a given DNA phosphorus content of 0.0011g / g, illustrate the accuracy of the two methods are different, but not very clear . With the experimental data of other researchers found that the protein removal for comparison is not very satisfactory. Key words: chicken liver; DNA; phenol-chloroform; ultraviolet and visible; Phosphorus determinat