二代测序实验与测序原理.pptVIP

序列质量 附：Solexa 1.3以前的quality计算公式是: Quality计算： Q是用phred quality score的计算方式计算得到: p是对应的碱基call错的概率 计算得到的Q值是一个整数, 将这个Q值加上33或者64后再转换成ASCII字符 SSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSS......................................... ..........................XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX.......... ...............................IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII.......... .................................JJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJJ.......... LLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLLL........................................ !#$%()*+,-./0123456789:;=?@ABCDEFGHIJKLMNOPQR