利用响应面法优化ε-聚赖氨酸发酵培养基.PDF

微生物学通报 MAY 20, 2011, 38(5): 641−646 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 研究报告 利用响应面法优化 ε-聚赖氨酸 发酵培养基 * 张超 张克峰 侯书国 马永山 (山东建筑大学市政与环境工程学院 山东 济南 250101) 摘 要: 采用响应面法对白色链霉菌 ZC7 发酵合成 ε-聚赖氨酸的培养基进行优化研究。采用 Plackett-Burman 法对8 个因素进行了筛选, 结果表明, 葡萄糖、酵母膏和硫酸铵的浓度对ε-聚赖氨 酸产量影响较大。用最陡爬坡试验及Box-Behnken 设计进一步优化, 利用Design-Expert 软件进行二 次回归分析, 得到各因素的最佳浓度为: 葡萄糖37.22 g/L, 酵母膏6.9 g/L, 硫酸铵 6.55 g/L。在此优 化条件下ε-聚赖氨酸的产量达到8.11 g/L, 较单因素试验最高值提高24.6% 。 关键词: 白色链霉菌, ε-聚赖氨酸, 优化, 响应面 Medium optimization of ε-polylysine production by response surface analysis * ZHANG Chao ZHANG Ke-Feng HOU Shu-Guo MA Yong-Shan (School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan, Shandong 250101, China) Abstract: In order to enhance the concentration of ε-polylysine (ε-PL) with Streptomyces albulus ZC7, response surface methodology were used to optimize the fermentation medium. A Plackett-Burman de- sign was used to evaluate the influence of eight factors firstly. Results showed that the concentration of glucose, yeast extract and (NH ) SO played important roles in influencing the content of ε-PL. Then, 4 2 4 the path of steepest ascent and the Box-Behnken desi