293细胞造就.docVIP

Protocol for Growth of HEK293 Cells in Suspension *Always wear lab coat and gloves Buffers and Media (see also file called media_buffers.doc) Detergents: Dodecyl-?-1-maltoside (DM) and n-octyl-?-D-glucoside (OG) can be obtained from Anatrace (Maumee, OH). Protein A-Sepharose was from RepliGen (Cambridge, MA), CNBr-activated Sepharose 4B was from Pharmacia. DMEM (Dulbeccos modified Eagles medium): Standard DMEM: Standard DMEM medium HB-Gro was purchased from Irvine Scientific (Santa Ana, CA). Powder for 10 L will be dissolved in 9 L Millipore water (distilled water). Add sodium bicarb