第九章外源基因在宿主细胞中的.pptVIP

Chapter9: High-level expression of foreign genes §1.gene silence §2. expression systems of foreign gene §3.Isolating and purifying the product of foreign genes §1.gene silence(基因沉默) Gene Silencing ??外源基因存在于生物体内，并未丢失或损伤，但该基因不表达或表达量极低的现象。. Gene silencing can occur at either transcriptional level(TGS,Transcriptional Gene Silencing), or post-transcriptional level(PTGS, Post-Transcriptional Gene Silencing). TGS: Methylation Induced Repeat-Induced Gene Silencing(RIGC), position effect ,and so on PTGS: cosuppression, and so on Methylation Induced 一、外源基因在原核细胞中的表达 欲将外源基因在原核细胞中表达，必须满足以下条件： ① 通过