氨磷汀对人巨核细胞白血病细胞促分化作用的机制研究.pdf

2017 2 141 Chinese Journal of Drug Application and Monitoring, Vol.14, No.1, February 2017 Dami 1 2 3 1 4 5 3 1 1. 100800 2. 518033 3. 100853 4. 100853 5. 100853 -1 []Dami 1 mmol·L Dami 12 dRT-PCR Western blot 04 d8 d12 d GATA-1NF-E2 Dami RT-PCR Western blot GATA-1 NF-E2 NF-E2GATA-1 Dami NF-E2GATA-1 []Dami NF-E2 GATA-1 [ ]R979.1 [ ]A [ ]1672 8157(2017)01 0019 04 Mechanism research of amifostine on inducing the differentiation of human megakaryoblastic leukemia Dami cell line 1 2 3 1 4 5 3 1 CHI Xiao-hua , WANG Hai-ying , YANG Bo , TIAN Huan-huan , CAI Li-li , LIU Cui , LU Xue-chun , TONG Wei-hang (1. Department of Pharmacy, General Hospital of Chinese PLA Rocket Force, Beijing 100800, China; 2. Department of Stomatology, Shenzhen Hospital of TCM, Shenzhen 518033, China; 3. Department of Nanlou Hematology, Chinese PLA General Hospital, Beijing 100853, China; 4. Department of Nanlou Clinical Laboratory, Chinese PLA General Hospital, Beijing 100853, China; 5. Department of Nanlou Ultrasonics, Chinese PLA General Hospital, Beijing 100853, China) [ABSTRACT]Objective: To explore the mechanism of amifostine on inducing the differentiation of human megakaryoblastic leukemia Dami cell line. Methods: The human megakaryoblastic leukemia Dami cell line were exposed to amifostine (1 mmol·L-1) for 12 days. At different time points (0 4 d 8 d 12 d), the expression of megakaryocytic differentiation-associated transcription factors such as GATA-binding factor 1 (GATA-1) and nuclear factor erythroid 2 (NF-E2) were detected by RT-PCR and Western blot