一株丛枝菌根真菌的形态与分子鉴定-华南农业大学学报.PDF

第27 卷第4 期 华南农业大学学报 VoL27 , No.4 2∞6 年 10 月 JOUTIlal of Soulh China Agricultural University Oct. 2∞6 一株丛枝菌根真菌的形态与分子鉴定 龙良鲍姚青2 羊宋贞朱红惠1 (1广东省微生物研究所，广东省菌种保藏与应用重点实验室，广东广州 51∞170; 2 华南农业大学园艺学院，广东广州 51创42) 摘要:从广东酸性土壤分离得到一种编号为2-1A1 的丛枝菌根真菌.其抱子在土壤中单生，未见抱子果，椭圆形，黄 色至褐色，抱子较大(170μmx230μm) ，抱壁一层，厚约 11 阻，Melzers 反应为黄色其形态特征与臼omusjlavispo- rum 的特征描述相一致-提取其单抱子DNA ， Nested PCR 扩增其 185 rDNA 的 N531-AMl 区，经序列测定，并在Gene­ Bank 上比对，进行系统进化树分析，结果表明，2-1Al 与 Glomωsp. (DQ371674) 的亲缘关系最近，序列同源性为 98%. 分子鉴定的方法只能将其鉴定到球囊霉属 αomω. 关键词:球囊霉属;形态; 185 rDNA; 进化树;鉴定 中固分类号:ψ39.5 文献标识码:A 文章编号:1∞1-411X(2∞6)04-α)40-03 Identification of an Arbuscular Mycorrhizal Fungi by the Methods of Morphology and Molecule l 2 l l LONG Liang-kun ,YAO Qing ,YANG 50ng-zhen ,ZHU Hong-hui (1 Guangdong Institute of Microbiology , Gu皿gdong Provincial Key Laboratory of Microbal Culture Collection and Application , Guangzhou 51∞70 ， China;2 College of Horticulture , South China Agric. Univ. , Guangzhou 510642 ,China) Abstract: 5pores of an arhuscular mycorrhizal fungal which marked as 2-1 A1 were isolated from the acid soil in Guangdong Province. 咀le spores formed singly in ωil ， no sporocarp , ellipsoid , yellow to hrown in color. 5pore wall consisted by only one layer and 11μm 由ick. Melzers reaction was yellow. 5train 2-1A1 accorded with Glomus 卢即臼porum in morphology. DNA was extracted from a single spore 2-1A1 , then the N531-AM1 region of 185 rDNA was amplified specifically hy nested PCR. PCR prod