I型鸭肝炎病毒中国分离株的全基因组序列测定及分析.pdfVIP

I型鸭肝炎病毒中国分离株的全基因组序列测定及分析.pdf

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VIROLOGICA SINICA, October 2007, 22 (5):353-359 CLC number: S852.65 Document code: A Article ID: 1674-0769(2007)05-0353-07 Complete Genomic Sequence of a Chinese Isolate of Duck * Hepatitis Virus 1** 2 1 1 1 Guang-qing LIU , Fei WANG , Zheng NI , Tao YUN , Bin YU , 1 ** Jiong-gang HUANG and Jian-Ping CHEN (1. Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 2. College of Animal Science and Technology, Anhui Agriculture University, Hefei 230036, China) Abstract: The complete genomic sequence of Duck hepatitis virus 1 (DHV-1) ZJ-V isolate was sequenced and determined to be 7 691 nucleotides (nt) in length with a 5′-terminal un-translated region (UTR) of 626 nt and a 3′-terminal UTR of 315 nt (not including the poly(A) tail). One large open reading frame (ORF) was found within the genome (nt 627 to 7 373) coding for a polypeptide of 2 249 amino acids. Our data also showed that the poly (A) tail of DHV-1 has at least 22 A’s. Sequence comparison revealed significant homology (from 91.9% to 95.7%) between the protein sequences of the ZJ-V isolate and those of 21 reference isolates. Although DHV-1 has been classified as an unassigned virus in the Picornaviridae family, its genome showed some unique characteristics. DHV-1 contains 3 copies of the 2A gene and only 1 c

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