番茄红素抗突变作用的实验研究.doc

`7890-=番茄红素抗突变作用的实验研究 李　欣,张　静,杨　颖,黄俊明熊习昆 (广东省疾病预防控制中心毒理所，广东　广州 510300) Study on the Role of Lycopene Anti-mutation LI Xin, ZHANG Jing, YANG Ying,HUANG Jun-ming, XIONG Xi-kun (Lab of Genetic Toxicology, Center of Disease Control and Prevention of Guangdong Province, Guangzhou? 510300, Guangdong, China) 　　【摘要】 背景与目的： 研究番茄红素对突变剂诱导的遗传损伤的抑制作用。 材料与方法： 采用修改的小鼠骨髓细胞微核试验和Ames试验：按《食品安全性毒理学评价程序和方法》试验，计数微核数、回复突变菌落数，计算微核率，相对菌落数和抑制率。 结果：小鼠骨髓细胞微核试验中预先给予番茄红素的试验组与环磷酰胺阳性组比较微核率明显降低，差异有统计学意义（P0.01），番茄红素对环磷酰胺诱发小鼠胸骨骨髓微核的抑制率达到59％以上。番茄红素预处理突变剂的Ames试验中TA98、TA100均有相对回变菌落数的减少，与致突变物对照组比较差异有统计学意义（P0.01），且呈明显的剂量-反应关系；番茄红素与突变剂同时加入的Ames试验中只有TA98在140 μg/皿剂量组有抑制作用。 结论： 番茄红素能有效抑制突变剂诱导的遗传损伤，具有一定的拮抗突变的作用。 　　【关键词】 番茄红素； 微核； Ames试验 　　中图分类号： Q343　　　　文献标识码： A　　　　文章编号： 1004－616X(2006)04－0327－04 ? 　　【ABSTRACT】 BACKGROUND ＆ AIM: To study the inhibition of lycopene on the genetic damage induced by mutagens? MATERIAL AND METHODS： In accordance with the “Procedures and Methods for Toxicological Assessment on Food Safety”, and the modified micronucleus test and Ames test were used. The numbers of micronuclei and bacteria colonies were scored, then the micronucleus frequencies in polychromatic erythrocytes(fMPCE), relative bacteria colonies and the rates of inhibition were calculated.? RESULTS: To compare with the positive control group treated with CP(Cyclophosphamide), the fMPCE in test groups pretreated with lycopene was significantly decreased(P0.01). The rate of inhibition of lycopene on fMPCE induced by CP was over 59％. Compared with the positive group, the relative bacteria colonies of TA98、TA100 were reduced in Ames test in which the mutagens were pretreated by lycopene, and there was adose-related were responses. But only the relative bacteria colonies of TA98 showed significant rise at 140 μg/dish in Ames test in where the mutagens and lycopene were added at the same time. CONCLUSION: Lycopene could effectively inhibit the genetic damage induced by mutagens, and to some degree it could play an anti-mutati