Chromosome－副本.pdfVIP

Chromosome Res (2010) 18:821–831 DOI 10.1007/s10577-010-9158-3 Chromosome elimination by wide hybridization between Triticeae or oat plant and pearl millet: pearl millet chromosome dynamics in hybrid embryo cells Takayoshi Ishii Toshie Ueda Hiroyuki Tanaka Hisashi Tsujimoto Received: 28 July 2010 /Revised: 16 September 2010 /Accepted: 17 September 2010 /Published online: 16 October 2010 # Springer Science+Business Media B.V. 2010 Abstract Wide crossing is one of a number of anaphase, respectively. The cause of the chromo- practical methods that can be used to expand some elimination of wheat–pearl millet hybrid is not genetic variation in common wheat (Triticum malfunction of the kinetochores binding to the aestivum). However, in crosses between wheat and spindles but the malfunction of the sister chromatids distantly related species such as maize (Zea mays) segregation at anaphase especially of chromosome and pearl millet (Pennisetum glaucum), non-wheat arm. chromosomes are often eliminated from the hybrid during embryogenesis. In this study, we used pearl Keywords chromosome elimination . millet pollen to pollinate the pistils of a range of embryogenesis . wide cross . pearl millet . wheat . oat plants in the tribe Triticeae, as well as oat. Seven days after pollination, the dynamics of the pearl Abbreviations millet chromosomes in the embryos were observed 2,4-D 2,4-Dichlorophenoxy acetic acid using in situ hybridization, probing both the pearl GISH Genomic in situ hybridization millet genomic DNA and its centromere-specific FISH Fluorescence in situ hybridization repeats. In embryos from the crosses with oat, all DAPI 4′,6-Diamidino-2-phenyl