多次饱和光闪---高光强条件下Yield测量地修正手段.PDF

多次饱和光闪高光强条件下 Yield 测量的修正手段 —植物逆境生理生态研究方法专题系列 10 研究发现， Yield ( ⊿F/Fm’)和ETR测量结果在高光强条件下存在高达 -41%的偏差(Loriaux S.D. 2006) ！！！ 从1990年开始，越来越多的研究表明，在 There has been increasing evidence since 高强度的光化光条件下，Yield和ETR测量结 1990 (Markgraf 1990) (Earl 2004) 果与气体交换参数（碳同化）测量结果不再 (Rosenqvist and vanKooten 2006), that at 具有良好的相关性；甚至在强烈的饱和光闪 high actinic light levels, quantum 情况下测得的荧光参数Yield和ETR ，与气体 photosynthetic yield, and ETR 交换测量参数相比，结果偏低(Markgraf 1990) measurements did not correlate well with (Earl 2004) (Rosenqvist and vanKooten gas exchange carbon assimilation 2006) 。这被视为非光化学荧光淬灭的一种表 measurements. Even with very intense 现形式，可能是因为高光强条件或者强烈的 saturation pulses, chlorophyll fluorescent 饱和光闪阻止了所有光反应中心的关闭(Earl quantum photosynthetic yield and ETR 2004) 。 measurements were found to underestimate values measured by gas exchange. It is believed to be caused by a form of non-photochemical quenching that prevents closure of all reaction centers in high light conditions even with the most intense saturation pulse (Earl 2004). As a solution, Earl (2004) found that he was 为了找出原