PCR检测儿童微小残留白血病的研究进展论文.docVIP

　　PCR检测儿童微小残留白血病的研究进展论文 【摘要】 微小残留白血病(MRD)是指白血病经化疗缓解后在骨髓中仍存在形态上不能检测到的白血病细胞，是白血病复发的主要原因。儿童MRD的检测对判断儿童白血病的预后、制定白血病的治疗方案有重要意义。PCR方法在MRD检测中具有快速、特异、简便、经济和样本量少等特点。本文综述近年以多重PCR初筛白血病靶基因，以荧光定量PCR（FQPCR）追踪MRD方面所取得的进展。 【关键词】 微小残留白血病 PCR 多重PCR FQPCR 儿童白血病 Detection of Minimal Residual Disease in Children Leukemia Patients by Using PCR ——Revieia has a potential importance to predict clinical oute and to modify treatment protocols of the diseases. Although some patients ia have achieved plete remission according to the clinical and morphological criteria, there are still very lobers of malignant cells that can not be discriminated by morphology and remained in bone marroinimal residual disease (MRD) and is the main reason leading to relapse. MRD detection has an important significance for designing treatment protocols. Several methods of MRD detection have been developed. These include conventional cytogeics, fluorescence in situ hybridization (FISH), floetric immunophenotyping (FCM), Southern blot and polymerase chain reaction (PCR) techniques, etc. Each of these techniques has its advantages and disadvantages, so not all of them are suitable for clinical MRD detection because of several inherent disadvantages, such as limited sensitivity, timeconsuming, high cost, or requiring highquality DNA or RNA. For example, the sensitivities of conventional cytogeics,.freelonitoring are 10-1-10-2, 10-2,10-3-10-4 and 10-1, respectively. Relatively, PCR can reach a good sensitivity of 10-4-10-6, and shoore advantages, such as fast, specific, simple and loinimal amounts of DNA or RNA for detection, etc., so PCR has its specific features for MRD detection. In this revieia specific marker by muitiplex PCR and FQPCR in recent years are summarized. Key inimal residual disease; PCR; multiplex PCR; FQPCR; children leukemia J Exp Hematol 2007; 15(3):652-656 微小残留白血病（minimal residual disease， MRD）是指白血病经化疗取得完全缓解后，骨髓中仍存在形态上不能检测到的白血病细胞，是引起白血病复发的主要原因。准确测定MRD对临床制定治疗方案具有重要意义。目前用于检测MRD的方法有核型分析、荧光原